Measurement of cell-cycle phase-specific cell death using Hoechst 33342 and propidium iodide: preservation by ethanol fixation.
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چکیده
منابع مشابه
Cell Cycle Specific Toxicity of the Hoechst 33342 Stain in Untreated or Irradiated Murine Tumor Cells1
Hoechst 33342 stain has been used previously in conjunction with fluorescence activated cell sorting to separate cell populations into the various phases of the cell cycle. The present experiments were designed to evaluate the toxicity of this stain in cells derived from solid kill sarcomas which were untreated or irradiated prior to dissociation. In both untreated and irradiated cells, stain t...
متن کاملCell cycle specific toxicity of the Hoechst 33342 stain in untreated or irradiated murine tumor cells.
Hoechst 33342 stain has been used previously in conjunction with fluorescence activated cell sorting to separate cell populations into the various phases of the cell cycle. The present experiments were designed to evaluate the toxicity of this stain in cells derived from solid KHT sarcomas which were untreated or irradiated prior to dissociation. In both untreated and irradiated cells, stain to...
متن کاملEffects of Hoechst 33342 on C2C12 and PC12 cell differentiation.
Accumulative evidence demonstrates that normal as well as cancer stem cells can be identified as a side population following Hoechst 33342 staining and flow cytometric analysis. This popular method is based on the ability of stem cells to efflux this fluorescent vital dye. We demonstrate that Hoechst 33342 can affect cell differentiation, suggesting potential complications in the interpretation...
متن کاملRapid flow cytofluorometric analysis of mammalian cell cycle by propidium iodide staining
A rapid method for the flow microfluorometric determination of the DNA content per cell is described. Incubation of cells in a hypotonic solution of propidium iodide results in disruption of the cell membrane and rapid staining of nuclear chromatin. DNA distribution histograms generated from cells stained by this method are identical to those generated after fixation and RNase digestion. In con...
متن کاملA pitfall of propidium iodide staining in fluorescence-activated cell sorting cell cycle analysis?
We read with great interest the recent article by Fischer et al. (1) on uracil-DNA glycosylase and its expression modulated by fluorodeoxyuridine (FdUrd). As a particular observation, the authors provide evidence that FdUrd-induced uracil-DNA glycosylase degradation occurred in late G1-early S transition instead of late S-G2-M phase as was observed previously in normally cycling cells (2). This...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1988
ISSN: 0022-1554,1551-5044
DOI: 10.1177/36.9.2457047